Structure of iodoform

• Article • • 29 May 2023 Cochlear transcript diversity and its role in auditory functions implied by an otoferlin short isoform • • • • • • • • • • ORCID: orcid.org/0000-0002-3992-2139 • • … • ORCID: orcid.org/0009-0000-7340-9913 Show authors Nature Communications volume 14, Article number: 3085 ( 2023) Isoforms of a gene may contribute to diverse biological functions. In the cochlea, the repertoire of alternative isoforms remains unexplored. We integrated single-cell short-read and long-read RNA sequencing techniques and identified 236,012 transcripts, 126,612 of which were unannotated in the GENCODE database. Then we analyzed and verified the unannotated transcripts using RNA-seq, RT-PCR, Sanger sequencing, and MS-based proteomics approaches. To illustrate the importance of identifying spliced isoforms, we investigated otoferlin, a key protein involved in synaptic transmission in inner hair cells (IHCs). Upon deletion of the canonical otoferlin isoform, the identified short isoform is able to support normal hearing thresholds but with reduced sustained exocytosis of IHCs, and further revealed otoferlin functions in endocytic membrane retrieval that was not well-addressed previously. Furthermore, we found that otoferlin isoforms are associated with IHC functions and auditory phenotypes. This work expands our mechanistic understanding of auditory functions at the level of isoform resolution. RNA expression at the isoform level is biologically more informative than at the ...

Acetaldehyde - C 2H 4O What is Acetaldehyde (CH3CHO)? C 2H 4O is an organic chemical compound with the chemical name Acetaldehyde. Acetaldehyde is also called MeCHO. It is miscible with napthalene, gasoline, xylene, ether, turpentine, alcohol and benzene. It has no colour and is a flammable liquid. It has a suffocating smell. It is non-corrosive to many metals but when It has a narcotic action and can cause mucous irritation. Table of Contents • • • • • • • • Acetaldehyde is widely used in the manufacturing of perfumes, drugs, Acetaldehyde Structure (C 2H 4O Structure) Acetaldehyde Formula The formula for Acetaldehyde CH 3CHO or C 2H 4O. Properties of Acetaldehyde – C 2H 4O C 2H 4O Acetaldehyde Molecular Weight/ Molar Mass 44.05 g/mol Density 0.784 g/cm 3 Boiling Point 20.2 °C Melting Point -123.5 °C Preparation of Acetaldehyde – CH 3CHO 1. Oxidation of Ethyl alcohol Ethyl alcohol (CH 3CH 2OH) undergoes oxidation in the presence of acidified K 2Cr 2O 7 or aqueous/alkaline KMnO 4 produces acetaldehyde. \(\begin \) 3. Rosenmund’s Reduction. Acetaldehyde can be prepared by the reduction of acetyl chloride with H 2 in the presence of palladium deposited over barium sulphate along with a small amount of salt of sulphur or quinoline. This mixture is known as Lindlar’s catalyst. This reaction is called Rosenmund’s Reduction. \(\begin \) In this reaction, CuCl 2 Converted Pd back into PdCl 2. 2CuCl 2 + Pd → PdCl 2 + 2CuCl Cuprous chloride is reoxidised to CuCl 2 by atmosph...

Iodoform is a yellow, crystalline solid belonging to the family of organic halogen compounds. It is also known as triiodomethane. What is a halogen, you may ask? Any of the six nonmetallic elements that comprise Group 17 of the periodic table are halogens. Thus, the halogen elements are fluorine, chlorine, bromine, iodine, astatine and Tennessee. Iodoform is an organoiodine compound with the formula – CHl 3 . It is a pale yellow, crystalline, volatile substance with a penetrating and distinctive odour. The use of iodoform is typical as a disinfectant. History of Iodoform George Simon Serullas first described the preparation of iodoform in the year 1822. The synthesis’s display was due to iodine vapour’s reactions with steam over red hot coals and potassium response with ethanolic iodine in water . You can prepare Iodoform by electrolysis of aqueous solutions containing acetone, inorganic iodides, and sodium carbonate. Several reagents convert iodoform to methylene iodide – a dense liquid, colourless when pure but usually discoloured by iodine traces – used as a heavy medium in gravity separation processes. The world first discovered iodoform’s antiseptic properties in 1880, whence it became an essential medicinal tool. But, currently, it has been replaced by more effective substances. Characteristics of Iodoform Let us now understand some characteristics of iodoform. • The electrolysis of an aqueous solution containing acetone, inorganic iodides, and sodium carbonate leads...